HEK293 human embryonic kidney cell line was stained with Rat Anti-Human FoxJ1 Monoclonal Antibody (Catalog # MAB3619, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human FoxJ1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxJ1

Human FoxJ1 (also HNF-4) is a 45 kDa, class 1 member of the HNF-3/fork-head gene family of transcription factors. It is 421 amino acids (aa) in length and contains one fork-head DNA binding domain (aa 120‑210). The presence of basic residues in the fork-head domain makes FoxJ1 a class 1 Fox protein. FoxJ1 induces cilia in lung and oviduct and maintains T cell tolerance to self-antigens. It is known to modulate NF kappaB activity. Over the range of amino acids used for immunization, human FoxJ1 is 89% aa identical to dog FoxJ1 and 87% aa identical to both mouse and rat FoxJ1.

Long Name

Forkhead Box J1

Alternate Names

FKHL13, HFH-4

Entrez Gene IDs

2302 (Human); 15223 (Mouse); 116557 (Rat)

Gene Symbol

FOXJ1

UniProt

Q92949.3

Additional FoxJ1 Products

Product Documents for Human FoxJ1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FoxJ1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human FoxJ1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3619

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FoxJ1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FoxJ1 Antibody

Detection of FoxJ1 in HEK293 Human Cell Line by Flow Cytometry.