Human FGF-19 Antibody

FGF‑19 in Human Prostate Cancer Tissue.

FGF-19 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human FGF-19 Monoclonal Antibody (Catalog # MAB969) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of d2uktu72gwvnainbyzhf.jpg by Immunohistochemistry

FGF19 is expressed focally within proliferative areas of embryonal hepatoblastoma.a Normalized counts of FGF19 detected by Smart-3SEQ. b Spatial expression of FGF19 in HB4 by 10x Visium. Adjusted p-values determined in CellRanger by negative binomial test with Benjamini-Hochberg adjustment for multiple hypothesis testing. c Quantification of FGF19+ spots/total spots in each cluster. d RNA in situ hybridization in primary hepatoblastoma HB15 detecting FGF19 (red) at low and high magnification. White dashed lines outline cells expressing FGF19. e Representative RNA in situ hybridization in serial sections of primary hepatoblastoma HB4 detecting FGF19, AXIN2, and SOX4 in red. White dashed lines outline cells expressing FGF19. Similar results obtained for n = 4 different patient specimens. f Double RNA in situ hybridization of AXIN2 (blue) / FGF19 (red), SOX4 (blue) / FGF19 (red) and FGF19 (blue) / KLB (red) in HB15 at low and high magnification. Similar results obtained for n = 4 different patient specimens. g Quantification of cells expressing AXIN2 (n = 5 images, 3074 cells), SOX4 (n = 6 images, 1441 cells), or KLB (n = 3 images, 1935 cells) that also co-express FGF19 as detected in f. Mean and s.d, ordinary one-way ANOVA with Tukey’s correction for multiple comparisons. h Quantification of cells expressing FGF19 that also co-express AXIN2 (n = 5 images, 84 cells), SOX4 (n = 6 images, 161 cells), or KLB (n = 3 images, 101 cells) as detected in f. Mean and s.d., ordinary one-way ANOVA with Tukey’s correction for multiple comparisons. For all panels – scale bar: 50 μm unless otherwise indicated, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ns: not significant. Source data are provided as a Source Data file. See also Supplementary Fig. S2, S3, S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39567523), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FGF-19 by Immunohistochemistry

FGF19 is expressed focally within proliferative areas of embryonal hepatoblastoma.a Normalized counts of FGF19 detected by Smart-3SEQ. b Spatial expression of FGF19 in HB4 by 10x Visium. Adjusted p-values determined in CellRanger by negative binomial test with Benjamini-Hochberg adjustment for multiple hypothesis testing. c Quantification of FGF19+ spots/total spots in each cluster. d RNA in situ hybridization in primary hepatoblastoma HB15 detecting FGF19 (red) at low and high magnification. White dashed lines outline cells expressing FGF19. e Representative RNA in situ hybridization in serial sections of primary hepatoblastoma HB4 detecting FGF19, AXIN2, and SOX4 in red. White dashed lines outline cells expressing FGF19. Similar results obtained for n = 4 different patient specimens. f Double RNA in situ hybridization of AXIN2 (blue) / FGF19 (red), SOX4 (blue) / FGF19 (red) and FGF19 (blue) / KLB (red) in HB15 at low and high magnification. Similar results obtained for n = 4 different patient specimens. g Quantification of cells expressing AXIN2 (n = 5 images, 3074 cells), SOX4 (n = 6 images, 1441 cells), or KLB (n = 3 images, 1935 cells) that also co-express FGF19 as detected in f. Mean and s.d, ordinary one-way ANOVA with Tukey’s correction for multiple comparisons. h Quantification of cells expressing FGF19 that also co-express AXIN2 (n = 5 images, 84 cells), SOX4 (n = 6 images, 161 cells), or KLB (n = 3 images, 101 cells) as detected in f. Mean and s.d., ordinary one-way ANOVA with Tukey’s correction for multiple comparisons. For all panels – scale bar: 50 μm unless otherwise indicated, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ns: not significant. Source data are provided as a Source Data file. See also Supplementary Fig. S2, S3, S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39567523), licensed under a CC-BY license. Not internally tested by R&D Systems.