Human Fetuin A/AHSG Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1184

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BAF1184
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Fetuin A/AHSG Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fetuin A

Specificity

Detects human Fetuin A in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity is observed with rmFetuin A.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Fetuin A/AHSG Biotinylated Antibody

Human Fetuin A / AHSG ELISA Standard Curve

Human Fetuin A / AHSG ELISA Standard Curve

Recombinant Human Fetuin A/AHSG (Catalog # 1184-PI) was serially diluted and captured by Mouse Anti-Human Fetuin A/AHSG Monoclonal Antibody (Catalog # MAB11841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Fetuin A/AHSG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1184) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Fetuin A/AHSG Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Fetuin A/AHSG (Catalog # 1184-PI)

Human Fetuin A/AHSG Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Fetuin A/AHSG Antibody (Catalog # MAB11841)
  • Standard: Recombinant Human Fetuin A/AHSG Protein, CF (Catalog # 1184-PI)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Human Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (1, 2). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (3). The major form of plasma Fetuin A corresponds to a disulfide bond-linked two chains derived from the single chain (4). Human Fetuin A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300 - 600 μg/mL), which fall significantly (30 - 50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (6, 7). The purified rhFetuin A corresponds to the single chain, which can be converted to the two-chain form by rhFurin (R&D Systems, Catalog # 1503-SE) in vitro. However, the conversion does not enhance its inhibitory activity against rhCathpesin V, a cysteine protease.

References

  1. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  2. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  3. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  4. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta. 671:78.
  5. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  6. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  7. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.

Long Name

alpha-2-HS Glycoprotein

Alternate Names

AHSG, alpha-2-HS-glycoprotein

Entrez Gene IDs

197 (Human); 11625 (Mouse); 25373 (Rat)

Gene Symbol

AHSG

Additional Fetuin A/AHSG Products

Product Documents for Human Fetuin A/AHSG Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Fetuin A/AHSG Biotinylated Antibody

For research use only

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