Human eNOS Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF950
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Rabbit
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human eNOS peptide
aa 1030-1203
aa 1030-1203
Specificity
Detects human eNOS.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human eNOS Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human placenta subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
0.1 µg/mL
Sample: Recombinant Human eNOS
Sample: Recombinant Human eNOS
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eNOS
Nitric oxide (NO) is a pleiotropic signaling molecule implicated in diverse biological processes including inhibition of platelet aggregation, regulation of neurotransmission, vasodilation, immune responses, and inflammation. NO is synthesized from L-arginine and O2 by three nitric oxide synthase (NOS) enzymes termed endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS). Each enzyme isoform is expressed in a variety of tissues and cell types. While eNOS and nNOS generally exhibit constitutive expression and are involved in physiological signaling and maintenance functions, iNOS expression is induced by inflammatory stimuli and is associated with both normal and pathological immune responses.
Long Name
Endothelial Nitric Oxide Synthase
Alternate Names
NOS3
Gene Symbol
NOS3
Additional eNOS Products
Product Documents for Human eNOS Biotinylated Antibody
Product Specific Notices for Human eNOS Biotinylated Antibody
For research use only
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