Human Cyclin D1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4314
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunocytochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 328303
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385
Met1-Ile295
Accession # P24385
Specificity
Detects human Cyclin D1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cyclin D1 Antibody
Cyclin D1 in COLO 205 Human Cell Line.
Cyclin D1 was detected in immersion fixed COLO 205 human colorectal adenocarcinoma cell line using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB4314) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Cyclin D1 by Western Blot
LPS suppressed EPCs cell viability via inducing cell pyroptosis. (a, b) NLRP3 was silenced in EPCs cells. (c) MTT assay was used to examine cell viability. (d) The expression status of Cyclin D1 and CDK2 were determined by Western blot analysis. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Cyclin D1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed COLO 205 human colorectal adenocarcinoma cell line
Sample: Immersion fixed COLO 205 human colorectal adenocarcinoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Reviewed Applications
Read 2 reviews rated 4 using MAB4314 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin D1
Cyclin D1 is a 44 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.
Alternate Names
Bcl-1, CCND1, Cyl-1, PRAD1
Gene Symbol
CCND1
UniProt
Additional Cyclin D1 Products
Product Documents for Human Cyclin D1 Antibody
Product Specific Notices for Human Cyclin D1 Antibody
For research use only
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