Human CXCR4 PE-conjugated Antibody

Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB173P) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) for 15 minutes at 37°C followed by 15 minutes at room temperature. View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR4 by Flow Cytometry

Representative example of flow cytometry quantification of complexes neutrophil–CD41a+ (NPAs, neutrophil–platelet aggregates) and lymphocyte markers’ expression. (A) Neutrophil cluster identification (region R1) based on its low CD14 expression (FL3) and side-scattering (SSC) morphological characteristics. (B) The R1-based histogram’s subtraction analysis (positive events (continuous line) minus isotype control (dotted line)) was used to quantify both the percentage of complexes CD41a+ (grey events in M1) and their RFI (median of M1 histogram minus median of the isotype control). (C) Lymphocyte cluster identification based on its forward- (FSC) and side-scattering characteristics (region R1). (D) Representative quantification of lymphocyte CXCR4 expression following the overlay histogram’s subtraction analysis (see above). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36836785), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of CXCR4 by Flow Cytometry

Representative example of flow cytometry quantification of complexes neutrophil–CD41a+ (NPAs, neutrophil–platelet aggregates) and lymphocyte markers’ expression. (A) Neutrophil cluster identification (region R1) based on its low CD14 expression (FL3) and side-scattering (SSC) morphological characteristics. (B) The R1-based histogram’s subtraction analysis (positive events (continuous line) minus isotype control (dotted line)) was used to quantify both the percentage of complexes CD41a+ (grey events in M1) and their RFI (median of M1 histogram minus median of the isotype control). (C) Lymphocyte cluster identification based on its forward- (FSC) and side-scattering characteristics (region R1). (D) Representative quantification of lymphocyte CXCR4 expression following the overlay histogram’s subtraction analysis (see above). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36836785), licensed under a CC-BY license. Not internally tested by R&D Systems.