Human CXCL11/I-TAC Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB672
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Development, Immunodepletion
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 87328
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL11/I-TAC
Phe22-Phe94
Accession # O14625
Phe22-Phe94
Accession # O14625
Specificity
Detects human CXCL11/I-TAC in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL5, 7, 8, 9, or 10. In Western blots, this antibody shows less than 1% cross-reactivity with rhCXCL1, 2, 3, 5, 6, 7, IL-8/CXCL8, 9, 10, 13, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, rmCXCL1, 2, 6, 9, 10, CXCL12/SDF-1 alpha, rmCXCL13, rrCXCL1, CXCL3/CINC-2 alpha, CXCL3/CINC-2 beta, or rpIL-8/CXCL8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCL11/I-TAC Antibody
Chemotaxis Induced by CXCL11/I-TAC and Neutralization by Human CXCL11/I-TAC Antibody.
Recombinant Human CXCL11/I-TAC (672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND50 is typically 0.0400-0.480 µg/mL.
Detection of CXCL11/I-TAC by Western Blot
CXCL10 cleavage by GP63 occurs between positions A81 and I82.(B) GP63 selectively cleaves chemokine ligands of the CXCR3 receptor. Conditioned media from L. major WT, deltagp63, and deltagp63+1 was incubated with human recombinant chemokines for 12 h and product detected by western blot. Cleavage is only detected for the CXCL9/10/11 family. Representative blots are shown from at least two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31440475), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human CXCL11/I-TAC Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)
Sample: Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)
Neutralization
Measured by its ability to neutralize CXCL11/I-TAC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.0400-0.480 μg/mL in the presence of 15 ng/mL Recombinant Human CXCL11/I-TAC.
Human CXCL11/I-TAC Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL11/I-TAC
CXCL11, also known as I-TAC, SCYB9B, H174 and beta-R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.
References
- Cole, K. et al. (1998) J. Exp. Med. 187:2009.
- Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
- Lou, Y. et al. (1998) J. Neurovirol. 4:575.
Alternate Names
beta-R1, H174, I-TAC, ITAC, SCYB9B
Gene Symbol
CXCL11
UniProt
Additional CXCL11/I-TAC Products
Product Documents for Human CXCL11/I-TAC Antibody
Product Specific Notices for Human CXCL11/I-TAC Antibody
For research use only
Loading...
Loading...
Loading...
Loading...