Detects human Complement Factor D in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhFactor II, rhFactor X, and rhFactor XI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Complement Factor D/Adipsin Biotinylated Antibody

Human Complement Factor D / Adipsin ELISA Standard Curve

Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE) was serially diluted and captured by Mouse Anti-Human Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB18241) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1824) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Complement Factor D/Adipsin Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE)

Human Complement Factor D/Adipsin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human Complement Factor D/Adipsin Antibody (Catalog # MAB18241)
Standard: Recombinant Human Complement Factor D Protein, CF (Catalog # 1824-SE)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF1824 in the following applications:

ELISA (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

References

White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
Taylor, F.R. et al. (1999) Biochemistry 38:2849.
Kim, S. et al. (1995) J. Biol. Chem. 270:24399.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

UniProt

P00746

Additional Complement Factor D/Adipsin Products

Product Documents for Human Complement Factor D/Adipsin Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Complement Factor D/Adipsin Biotinylated Antibody

For research use only

Citations
Reviews

Human Complement Factor D/Adipsin Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1824

Key Product Details

Species Reactivity

Validated:

Cited:

Applications