Human Collagen I alpha 1 MAb (Clone 816154)

R&D Systems, part of Bio-Techne | Catalog # MAB62202

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MAB62202-100
MAB62202-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 816154

View all Collagen I alpha 1 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human Collagen I alpha1
Gln23-Lys277 + Gly1094-Leu1464,
Accession # P02452

Specificity

Detects human Collagen I alpha1 in ELISAs. In direct ELISA, no cross-reactivity with human COL-2A1, COL-3A1 or COL-4A1 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Collagen I alpha 1 MAb (Clone 816154)

Human Collagen I  alpha1 ELISA Standard Curve.

Recombinant Human Collagen I  alpha1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Collagen I  alpha1 Monoclonal Antibody (Catalog # MAB62201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Collagen I  alpha1 Monoclonal Antibody (Catalog # MAB62202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Collagen I alpha 1 MAb (Clone 816154)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with mouse anti-Human Collagen I alpha1 monoclonal antibody (Catalog # MAB62201).This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen I alpha 1

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides (1). The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues (1). Type I collagen is a heterotrimer that consists of two alpha1(I) chains and one alpha2(I) chain, although homotrimers consisting of three identical alpha1(I) chains have also been described (2). This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N‑telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I( alpha1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.

References

  1. Canty, E.G. et al. (2005) J. Cell Sci. 118:1341.
  2. Han, S. et al. (2008) J. Mol. Biol. 383:122.

Alternate Names

COL1A1, OI4

Entrez Gene IDs

1277 (Human); 12842 (Mouse); 29393 (Rat)

Gene Symbol

COL1A1

UniProt

P02452

Additional Collagen I alpha 1 Products

Product Documents for Human Collagen I alpha 1 MAb (Clone 816154)

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Certificate of Analysis

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Product Specific Notices for Human Collagen I alpha 1 MAb (Clone 816154)

For research use only

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