Chromogranin B was detected in immersion fixed paraffin-embedded sections of human pituitary gland using Mouse Anti-Human Chromogranin B Monoclonal Antibody (Catalog # MAB8868) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Chromogranin B Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pituitary gland

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chromogranin B

Chromogranin B (CHGB), also known as Secretogranin-1, is a 673 amino acid neuroendocrine secretory granule protein which is cleaved into three fragments: PE-11 (aa 575-585), GAWK peptide (aa 440-513) and CCB peptide (617-673). Human CHGB was originally isolated from human pheochromocytoma tissue, and is expressed in normal and malignant neuroendocrine tissues. CHGB peptides play a role in hormonal regulation in pancreatic islets, hypothalamus and pituitary gland. CHGB peptides, (aa 314-332 and 350-365) have been identified in several endocrine tumors including, gastrinoma, thyroid carcinoma, insulinoma, pancreatic endocrine tumor and pituitary adenomas.

Alternate Names

CHGB, SCG1, Secretogranin I

Entrez Gene IDs

1114 (Human); 12653 (Mouse); 24259 (Rat)

Gene Symbol

CHGB

UniProt

P05060

Additional Chromogranin B Products

Product Documents for Human Chromogranin B Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Chromogranin B Antibody

For research use only

Citations
Reviews

Human Chromogranin B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8868

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Chromogranin B Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Chromogranin B Antibody

Chromogranin B in Human Pituitary Gland.