Human CD82/Kai-1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4616A

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 423524

View all CD82/Kai-1 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
Met1-Tyr267
Accession # AAH01821

Specificity

Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD82/Kai-1 APC-conjugated Antibody

Detection of CD82/Kai-1 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CD82/Kai‑1 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD82/Kai-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4616A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD82/Kai-1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD82/Kai-1

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Alternate Names

CD82, IA4, KAI1, SAR2, ST6, TSPAN27

Entrez Gene IDs

3732 (Human); 12521 (Mouse); 83628 (Rat)

Gene Symbol

CD82

UniProt

AAH01821

Additional CD82/Kai-1 Products

Product Documents for Human CD82/Kai-1 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD82/Kai-1 APC-conjugated Antibody

For research use only

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