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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 34930

Product Summary for Human CD4 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Extracellular domain

Specificity

Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD4 Antibody

CD4 antibody in Human PBMCs by Immunocytochemistry (ICC).

CD4 in Human PBMCs.

CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB379) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human CD4 by Immunocytochemistry/Immunofluorescence

Detection of Human CD4 by Immunocytochemistry/Immunofluorescence

CX3CR1 expressions in affected muscle of patients with polymyositis. The muscle tissues of polymyositis (PM) patients were double-stained with CD4, CD8, or CD68, as well as CX3CR1, and were analyzed with fluorescent microscopy: (A) CX3CR1, (B) CD4, (C) merged (A) and (B), (D) CX3CR1, (E) CD8, (F) merged (D) and (E), (G) CX3CR1, (H) CD68, and (I) merged (G) and (H). Arrows indicate double-positive cells. Original magnification, ×400. Image collected and cropped by CiteAb from the following publication (http://arthritis-research.biomedcentral.com/articles/10.1186/ar3761), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CD4 by Immunocytochemistry/Immunofluorescence

Detection of Human CD4 by Immunocytochemistry/Immunofluorescence

CX3CR1 expressions in affected muscle of patients with polymyositis. The muscle tissues of polymyositis (PM) patients were double-stained with CD4, CD8, or CD68, as well as CX3CR1, and were analyzed with fluorescent microscopy: (A) CX3CR1, (B) CD4, (C) merged (A) and (B), (D) CX3CR1, (E) CD8, (F) merged (D) and (E), (G) CX3CR1, (H) CD68, and (I) merged (G) and (H). Arrows indicate double-positive cells. Original magnification, ×400. Image collected and cropped by CiteAb from the following publication (http://arthritis-research.biomedcentral.com/articles/10.1186/ar3761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood lymphocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using MAB379 in the following applications:

Published Applications

Read 6 publications using MAB379 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) precursor protein with a 25 aa signal peptide, a 371 aa extracellular region containing four immunoglobulin homology domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

References

  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

Product Documents for Human CD4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD4 Antibody

For research use only

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FAQs for Human CD4 Antibody

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  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A: Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Reconstitution Calculator

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