Human CCRL2/CRAM-A/B APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB23501A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 152254
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CRAM-A
Met1-Val356 (Val180Met)
Accession # NP_001124382
Met1-Val356 (Val180Met)
Accession # NP_001124382
Specificity
Detects human CCRL2/CRAM‑A/B. Stains human CCRL2-transfected cells but not irrelevant transfectants. Detects both the CRAM-A and CRAM-B isoforms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CCRL2/CRAM-A/B APC-conjugated Antibody
Detection of CCRL2/CRAM‑A/B in PBMCs by Flow Cytometry.
Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of CCRL2/CRAM‑A/B in MDA-MB-231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human CCRL2/CRAM-A/B APC-conjugated Monoclonal Antibody (Catalog # FAB23501A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (IC0041A, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Human CCRL2/CRAM-A/B APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MDA-MB-231 human breast adenocarcinoma cell line or Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL)
Sample: MDA-MB-231 human breast adenocarcinoma cell line or Peripheral Blood Mononuclear Cells (PBMCs) cultured with Recombinant Human IL-2 (Catalog # 202-IL)
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: CCRL2/CRAM-A/B
References
- Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
- Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
- Hartmann, T.N. et al. (2008) Immunology 125:252.
- Leick, M. et al. (2010) Immunology 129:536.
Long Name
CC Chemokine Receptor-like 2
Alternate Names
ACKR5, CCRL2, CKRX, CRAM-A, CRAM-B, CRAMA, CRAMB, HCR
Entrez Gene IDs
9034 (Human)
Gene Symbol
CCRL2
UniProt
Additional CCRL2/CRAM-A/B Products
Product Documents for Human CCRL2/CRAM-A/B APC-conjugated Antibody
Product Specific Notices for Human CCRL2/CRAM-A/B APC-conjugated Antibody
For research use only
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