Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 40% cross‑reactivity with recombinant human (rh) HCC-1 is observed, less than 10% cross‑reactivity with rhEotaxin is observed, and less than 5% cross‑reactivity with rhMCP-1 is observed. Neutralizes the biological activity of rhCCL8. This antibody will not neutralize the biological activity of rhMCP-1, recombinant mouse JE, or rhMCP-3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL8/MCP-2 Antibody

Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/MCP‑2 Antibody.

Human CCL8/MCP‑2 Antibody (Catalog # AF-281-NA) neutralizes CCL8/MCP-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically <20.0 µg/mL in the presence of 250 ng/mL Recombinant Human CCL8/MCP-2.

Applications for Human CCL8/MCP-2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)

Neutralization

Measured by its ability to neutralize CCL8/MCP-2-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically <20.0 µg/mL in the presence of 250 ng/mL Recombinant Human CCL8/MCP-2.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL8/MCP-2

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Entrez Gene IDs

6355 (Human); 20307 (Mouse)

Gene Symbol

CCL8

UniProt

P80075

Additional CCL8/MCP-2 Products

Product Documents for Human CCL8/MCP-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCL8/MCP-2 Antibody

For research use only

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