Human CCL4/MIP-1 beta PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC271C
Discontinued Product
IC271C has been discontinued.
View all CCL4/MIP-1 beta products.
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 24006
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
Ala24-Asn92
Accession # P13236
Ala24-Asn92
Accession # P13236
Specificity
Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CCL4/MIP-1 beta PerCP-conjugated Antibody
Detection of CCL4/MIP‑1 beta in LPS-treated Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes, resting (open histogram), or treated with LPS (filled histogram), were stained with Mouse Anti-Human CCL4/MIP-1 beta PerCP-conjugated Monoclonal Antibody (Catalog # IC271C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human CCL4/MIP-1 beta PerCP-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: Human peripheral blood monocytes treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCL4/MIP-1 beta
Alternate Names
Exodus-3, MIP-1 beta, MIP1 beta
Entrez Gene IDs
Gene Symbol
CCL4
UniProt
Additional CCL4/MIP-1 beta Products
Product Documents for Human CCL4/MIP-1 beta PerCP-conjugated Antibody
Product Specific Notices for Human CCL4/MIP-1 beta PerCP-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...