Detects human CCL15/MIP-1 delta in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human MPIF‑1, recombinant mouse (rm) C10, and rmMIP-1 gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CCL15/MIP‑1 delta Biotinylated Antibody

Human CCL15 / MIP-1 delta ELISA Standard Curve

Human CCL15/MIP-1 delta was serially diluted and captured by Mouse Anti-Human CCL15/MIP-1 delta Monoclonal Antibody (Catalog # MAB363) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL15/MIP-1 delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL15/MIP‑1 delta Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)

Human CCL15/MIP-1 delta Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human CCL15/MIP-1 delta Antibody (Catalog # MAB363)
Standard: Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein (Catalog # 363-MG)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL15/MIP-1 delta

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1 delta is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

References

Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Alternate Names

HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3

Entrez Gene IDs

6359 (Human)

Gene Symbol

CCL15

UniProt

Q16663

Additional CCL15/MIP-1 delta Products

Product Documents for Human CCL15/MIP‑1 delta Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCL15/MIP‑1 delta Biotinylated Antibody

For research use only

Citations
Reviews

Human CCL15/MIP‑1 delta Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF363

Key Product Details

Species Reactivity

Validated:

Cited:

Applications