Detects human CCL14/HCC‑1/HCC‑3 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 6, 7, 9, 11, 12, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL14/HCC-1/HCC-3 Antibody

Chemotaxis Induced by CCL14a/HCC‑1 and Neutral-ization by Human CCL14/ HCC‑1/HCC‑3 Antibody.

Recombinant Human CCL14a/ HCC-1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCL14/HCC-1/ HCC-3 Monoclonal Antibody (Catalog # MAB3241). The ND₅₀ is typically 0.5-2.5 µg/mL.

Human CCL14a / HCC-1 ELISA Standard Curve

Recombinant Human CCL14a/HCC-1 aa 20-93 (Catalog # 324-HC) was serially diluted and captured by Rat Anti-Human CCL14/HCC-1/HCC-3 Monoclonal Antibody (Catalog # MAB3241) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL14a/HCC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-324-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL14/HCC-1/HCC-3 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL14a/HCC-1 aa 20-93 (Catalog # 324-HC)

Neutralization

Measured by its ability to neutralize CCL14a/HCC-1 -induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL in the presence of 10 ng/mL Recombinant Human CCL14a/HCC-1 aa 28-93.

Human CCL14a/HCC-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human CCL14a/HCC‑1 Biotinylated Antibody (Catalog # BAF324)
Standard: Recombinant Human CCL14a/HCC-1 Protein (Catalog # 324-HC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 3.8 using MAB3241 in the following applications:

Block/Neutralize (1 Review)
Microarray (1 Review)
Microarrays (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL14/HCC-1/HCC-3

CCL14 is a chemokine that promotes chemotaxis of T cells, monocytes, and eosinophils. It occurs in two isoforms resulting from differential mRNA splicing. Following cleavage of a 19 amino acid signal peptide, mature CCL14a (aa 20‑93) is a 74 amino acid peptide that is also known as HCC-1 (Hemofiltrate CC Chemokine-1). It is a weak CCR1 agonist, however, an 8 amino acid N-terminal truncation (aa 28‑93) allows potent signaling through CCR1 and CCR5. CCL14b, also known as HCC-3, is a 90 amino acid peptide (aa 20‑109) resulting from the insertion of 16 amino acids between residues 7 and 8 of CCL14a.

Alternate Names

C-C motif chemokine 14, chemokine (C-C motif) ligand 14, Chemokine CC-1/CC-3, chemokine CC-3, CKB1, FLJ16015, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, hemofiltrate CC chemokine 1, MCIF, member 14, NCC2CC-1, NCC-2CKb1, new CC chemokine 2, SCYA14CC-3, Small-inducible cytokine A14

Entrez Gene IDs

6358 (Human)

Gene Symbol

CCL14

UniProt

NP_116738

Additional CCL14/HCC-1/HCC-3 Products

Product Documents for Human CCL14/HCC-1/HCC-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCL14/HCC-1/HCC-3 Antibody

For research use only

Citations
Reviews

Human CCL14/HCC-1/HCC-3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3241

Key Product Details

Species Reactivity

Validated:

Cited:

Applications