Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Cathepsin V Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin V
Val18-Val334
Accession # O60911

Specificity

Detects human Cathepsin V in Western blots. In this format, approximately 15% cross-reactivity with recombinant human (rh) Cathepsin C, rhCathepsin A, rhCathepsin D, rhCathepsin O is observed and less than 5% cross-reactivity with rhCathepsin S and rhCathepsin X/Z/P is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Cathepsin V Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cathepsin V (Catalog # 1080-CY)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin V

Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.

References

  1. Turk, V. et al. (2001) EMBO J. 20:4629.
  2. Bromme, D. et al. (1999) Biochemistry 38:2377.
  3. Somoza, J.R. et al. (2000) Biochemistry 39:12543.

Alternate Names

Cathepsin L2, Cathepsin U, CTSL2

Entrez Gene IDs

1515 (Human)

Gene Symbol

CTSV

UniProt

O60911

Additional Cathepsin V Products

Product Documents for Human Cathepsin V Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cathepsin V Biotinylated Antibody

For research use only

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