Detects human Cathepsin L in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse Cathepsin L, recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin E, rhCathepsin S, and rhCathepsin V is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cathepsin L Biotinylated Antibody

Human Cathepsin L ELISA Standard Curve

Recombinant Human Cathepsin L (Catalog # 952-CY) was serially diluted and captured by Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF952) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Cathepsin L Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cathepsin L (Catalog # 952-CY)

Human Cathepsin L Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human Cathepsin L Antibody (Catalog # AF952)
Standard: Recombinant Human Cathepsin L Protein, CF (Catalog # 952-CY)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin L

Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage (1). Cathepsin L has also been shown to proteolytically inactivate alpha₁-antitrypsin and secretory leucoprotease inhibitor, two major protease inhibitors of the respiratory tract (2). These observations, combined with the demonstration of increased Cathepsin L activity in the epithelial lining fluid of the lungs of emphysema patients, have led to the suggestion that the enzyme may be involved in the progression of this disease. Cathepsin L has also been identified as a major excreted protein of transformed fibroblasts, indicating the enzyme could be involved in malignant tumor growth (3). Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5‑6.5. The stability of the enzyme decreases at higher pH values.

References

Goretzki, L. et al. (1992) FEBS Lett. 297:112.
Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
Gottesman, M.M. and F. Cabral (1981) Biochemistry 20:1659.

Alternate Names

CTSL, CTSL1

Entrez Gene IDs

1514 (Human); 13039 (Mouse); 118276442 (Insect)

Gene Symbol

CTSL

UniProt

P07711

Additional Cathepsin L Products

Product Documents for Human Cathepsin L Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cathepsin L Biotinylated Antibody

For research use only

Citations
Reviews

Human Cathepsin L Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF952

Key Product Details

Species Reactivity

Validated:

Cited:

Applications