Human BMP-4 Alexa Fluor™ Plus 405-conjugated MAb (Clone 66119)

R&D Systems, part of Bio-Techne | Catalog # FAB757AFP405

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FAB757AFP405-100UG
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 66119

View all BMP-4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP-4

Specificity

Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BMP-4

BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).

References

  1. Zhang, P. et al., 2008, Blood 111:1933.
  2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
  3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.
  5. Frank, D.B. et al., 2005, Circ. Res. 97:496.
  6. Wozney, J. et al., 1988, Science 242:1528.
  7. Cui, Y. et al., 1998, EMBO J. 17:4735.
  8. Cui, Y. et al., 2001, Genes Dev. 15:2797.
  9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
  11. Chen, D. et al., 2004, Growth Factors 22:233.
  12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
  13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
  15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Gene Symbol

BMP4

UniProt

P12644

Additional BMP-4 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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