Human Aquaporin 4/AQP4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11748
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3366C
Product Specifications
Immunogen
Synthetic Peptide
Accession # P55087
Accession # P55087
Specificity
Detects a synthetic peptide specific for human Aquaporin-4 around amino acid 315 in Direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Aquaporin 4/AQP4 Antibody
Detection of AQP4 in Human Brain Cortex via seqIF™ staining on COMET™
AQP4 was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11748) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH)Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder.
Detection of Aquaporin 4/AQP4 in Human Brain Cortex.
Aquaporin 4/AQP4 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Rabbit Anti-Human Aquaporin 4/AQP4 Monoclonal Antibody (Catalog # MAB11748) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Aquaporin 4/AQP4 Antibody
Application
Recommended Usage
Immunohistochemistry
1-10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Multiplex Immunofluorescence
10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Sample: Immersion fixed paraffin-embedded sections of human brain cortex
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aquaporin 4/AQP4
References
- Jung JS, Bhat RV, Preston GM, Guggino WB, Baraban JM, Agre P. Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):13052-6. doi: 10.1073/pnas.91.26.13052. PMID: 7528931; PMCID: PMC45579.
- Halsey AM, Conner AC, Bill RM, Logan A, Ahmed Z. Aquaporins and Their Regulation after Spinal Cord Injury. Cells. 2018 Oct 18;7(10):174. doi: 10.3390/cells7100174. PMID: 30340399; PMCID: PMC6210264.
- Verkman AS, Phuan PW, Asavapanumas N, Tradtrantip L. Biology of AQP4 and anti-AQP4 antibody: therapeutic implications for NMO. Brain Pathol. 2013 Nov;23(6):684-95. doi: 10.1111/bpa.12085. PMID: 24118484; PMCID: PMC3890327.
- Gleiser C, Wagner A, Fallier-Becker P, Wolburg H, Hirt B, Mack AF. Aquaporin-4 in Astroglial Cells in the CNS and Supporting Cells of Sensory Organs-A Comparative Perspective. Int J Mol Sci. 2016 Aug 26;17(9):1411. doi: 10.3390/ijms17091411. PMID: 27571065; PMCID: PMC5037691.
Long Name
Aquaporin 4
Alternate Names
AQP-4, AQP4, MIWC, WCH4
Entrez Gene IDs
361 (Human)
Gene Symbol
AQP4
UniProt
Additional Aquaporin 4/AQP4 Products
Product Documents for Human Aquaporin 4/AQP4 Antibody
Product Specific Notices for Human Aquaporin 4/AQP4 Antibody
For research use only
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