Western blot shows lysates of human plasma and liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein M/ApoM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein M/ApoM at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Human Apolipoprotein M/ApoM Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human plasma and liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein M/ApoM

Apolipoprotein M (ApoM; also known as Protein G3a) is a 25 kDa, secreted member of the lipocalin family of proteins. It is produced by hepatocytes and renal tubular epithelium, and represents a minor component of HDL particles. Human ApoM is 188 amino acids (aa) in length. It contains an uncleaved, hydrophobic N-terminal segment and one utilized N-linked glycosylation site (mouse ApoM shows no glycosylation). At least two potential isoforms exist. One shows an alternate start site at Met73, while a second shows the same start site plus a 21 aa substitution for aa 181‑188. Human ApoM shares 91% and 80% aa identity with pig and mouse ApoM, respectively.

Alternate Names

APOM, NG20, Protein G3a

Entrez Gene IDs

55937 (Human); 55938 (Mouse); 55939 (Rat)

Gene Symbol

APOM

UniProt

O95445

Additional Apolipoprotein M/ApoM Products

Product Documents for Human Apolipoprotein M/ApoM Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Apolipoprotein M/ApoM Antibody

For research use only

Citations
Reviews

Human Apolipoprotein M/ApoM Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4550

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Apolipoprotein M/ApoM Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Apolipoprotein M/ApoM Antibody

Detection of Human Apolipoprotein M/ApoM by Western Blot.