HspA6 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97536
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human HspA6 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for HspA6 Antibody - BSA Free
Western Blot: HspA6 Antibody [NBP2-97536]
Western Blot: HspA6 Antibody [NBP2-97536] - Anti-HspA6 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDaImmunocytochemistry/Immunofluorescence: HspA6 Antibody [NBP2-97536] -
Immunocytochemistry/Immunofluorescence: HspA6 Antibody [NBP2-97536] - Immunofluorescence staining of HspA6 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97536 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Applications for HspA6 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HSPA6/HSP70B
Long Name
Heat Shock Protein A6
Alternate Names
HSP70B
Gene Symbol
HSPA6
Additional HSPA6/HSP70B Products
Product Specific Notices for HspA6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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