HSP27 [p Ser78] Antibody (24GB4035)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50770

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NBP3-50770-100ul
NBP3-50770-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4035

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-HSP27 (S78) (Uniprot: P04792)

Modification

p Ser78

Specificity

This antibody detects endogenous levels of HSPB1 only when phosphorylated at S78

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP27 [p Ser78] Antibody (24GB4035)

HSP27 [p Ser78] Antibody (24GB4035)

Immunocytochemistry/ Immunofluorescence: HSP27 [p Ser78] Antibody (24GB4035)[NBP3-50770] -

Immunocytochemical staining of HepG2 cells with HSP27 antibody (1:1,000). Nuclei were stained blue with DAPI; HSP27 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
HSP27 [p Ser78] Antibody (24GB4035)

Flow Cytometry: HSP27 [p Ser78] Antibody (24GB4035)[NBP3-50770] -

Flow cytometric analysis of HSP27expression in HepG2 cells using HSP27 antibody (1:2,000). Green, isotype control; red, phospho-Hsp27 (S78).
HSP27 [p Ser78] Antibody (24GB4035)

Western Blot: HSP27 [p Ser78] Antibody (24GB4035)[NBP3-50770] -

Western blotting analysis using HSP27 (S78) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for HSP27 [p Ser78] Antibody (24GB4035)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 [p Ser78] Antibody (24GB4035)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP27 [p Ser78] Antibody (24GB4035)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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