HRV3c Recognition Site Antibody

Western Blot: HRV3c Recognition Site Antibody [NBP2-29904]

Western Blot: HRV3c Recognition Site Antibody [NBP2-29904] - Analysis of HRV3c performed on control protein containing GST & the HRV3c cleavage site. Antigen-antibody complexes: incubated 100ug HeLa lysate containing 2ug control protein with 4ug of an HRV3c pAb overnight on a rocking platform at 4C. Immune complexes were captured on 50ul of Protein A/G plus Agarose, washed extensively, & eluted with 5X Lane Marker Reducing Sample Buffer. Lysate & the immune complexes were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to PVDF membrane, & blocked with 5% BSA/TBS-0.1%Tween for 1 hr. Membrane was probed with GST monoclonal antibody at 1:1500 overnight rotating at 4C, washed in TBST, & probed with Clean-blot IP detection reagent at 1:2500 for at least 1 hr. Chemiluminescent detection performed using SuperSignal West Dura. Note: the IP fraction resulted in proteolytic activity of the GST-control protein.

Western Blot: HRV3c Recognition Site Antibody [NBP2-29904]

Western Blot: HRV3c Recognition Site Antibody [NBP2-29904] - Analysis of 1ug of a control protein, either uncleaved (lane 1) or cleaved (lane 2) using the Human Rhinovirus 3C Protease (HRV 3C) per well.