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HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08318

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # rHPV16L1/1058

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Human HPV16, major capsid protein L1

Reactivity Notes

Type 16 of human Papilloma Virus (HPV-16)

Localization

Nuclear

Specificity

Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07259).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free

Immunohistochemistry-Paraffin: HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free [NBP3-08318]

Immunohistochemistry-Paraffin: HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free [NBP3-08318]

Immunohistochemistry-Paraffin: HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free [NBP3-08318] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV16 Mouse Recombinant Monoclonal Antibody (rHPV16L1/1058).

Applications for HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HPV16

L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been asso ciated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

Alternate Names

Human Papilloma Virus Type 16

Additional HPV16 Products

Product Documents for HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HPV16 Antibody (rHPV16L1/1058) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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