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HPCL Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89384

Novus Biologicals, part of Bio-Techne
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NBP1-89384
NBP1-89384-25ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKFQDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HPCL Antibody - BSA Free (NBP1-89384) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HPCL Antibody - BSA Free

HPCL Antibody - BSA Free Immunohistochemistry-Paraffin: HPCL Antibody - BSA Free [NBP1-89384]

Immunohistochemistry-Paraffin: HPCL Antibody - BSA Free [NBP1-89384]

Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
HPCL Antibody - BSA Free Western Blot: HPCL Antibody - BSA Free [NBP1-89384]

Western Blot: HPCL Antibody - BSA Free [NBP1-89384]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
HPCL Antibody - BSA Free Western Blot: HPCL Antibody - BSA Free [NBP1-89384]

Western Blot: HPCL Antibody - BSA Free [NBP1-89384]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for HPCL Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPCL

HPCL catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde

Alternate Names

1600020H07Rik, 2,2-HPCL, 2-hydroxyacyl-CoA lyase 1, 2-hydroxyphytanoyl-CoA lyase2-hydroxyphytanol-CoA lyase, EC 4.1, HPCL, PHYH2FLJ55041, Phytanoyl-CoA 2-hydroxylase 2, phytanoyl-CoA hydroxylase 2

Gene Symbol

HACL1

Additional HPCL Products

Product Documents for HPCL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HPCL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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