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HMW E.Coli Host Cell Proteins Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44221

Novus Biologicals, part of Bio-Techne
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NBP2-44221
NBP2-44221-0.05ml

Key Product Details

Species Reactivity

E. coli

Applications

Dot Blot, ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).

Reactivity Notes

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for HMW E.Coli Host Cell Proteins Antibody - BSA Free

Western Blot: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221]

Western Blot: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221]

Western Blot: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221] - 2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 Ug HMW HCP.
ELISA: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221]

ELISA: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221]

ELISA: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221] - ELISA of Rabbit anti-High Molecular Weight Host Cell Protein Antigen: BSA conjugates of HMW HCP. Coating amount: 1 Ug per well titrated 1:3 in 1XPBS.

Applications for HMW E.Coli Host Cell Proteins Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:500-1:1000
Application Notes
This product detects over 250 E.coli HMW proteins and has been tested in 2D, western blotting, and ELISA.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HMW E.Coli Host Cell Proteins

Additional HMW E.Coli Host Cell Proteins Products

Product Documents for HMW E.Coli Host Cell Proteins Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMW E.Coli Host Cell Proteins Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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