HLA DQB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48501
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human HLA DQ1/DQ3. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HLA DQB1 Antibody
Western Blot: HLA DQB1 Antibody [NBP3-48501] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA DQB1 antibody (NBP3-48501) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry: HLA DQB1 Antibody [NBP3-48501] -
HLA DQB1 antibody detects HLA DQB1 protein at cytoplasm and membrane on human lung adenocarcinoma by immunohistochemical analysis.Sample: Paraffin-embedded human lung adenocarcinoma.
HLA DQB1 antibody (NBP3-48501) diluted at 1:500.
Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: HLA DQB1 Antibody [NBP3-48501] -
HLA DQB1 antibody detects HLA DQB1 protein at cell membrane and endoplasmic reticulum by immunofluorescent analysis.Sample: Raji cells were fixed in iced-cold MeOH for 5 min.
Green: HLA DQB1 protein stained by HLA DQB1 antibody (NBP3-48501) diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for HLA DQB1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLA DQB1
Alternate Names
DC-1 alpha chain, DC-alpha, HLA class II histocompatibility antigen, DQ alpha 1 chain-like, HLA-DCA, MHC class II DQA1
Gene Symbol
HLA-DQB1
Additional HLA DQB1 Products
Product Specific Notices for HLA DQB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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