HLA-ABCE Antibody (TP25.99SF) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68006

Novus Biologicals, part of Bio-Techne
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NBP2-68006
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TP25.99SF

Format

BSA Free

Concentration

1 mg/ml

View all HLA-ABCE Antibodies »

Product Specifications

Immunogen

IFN-gamma-treated human melanoma cells COLO 38.

Specificity

The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for distinguishing between HLA-G and other HLA-class I antigens.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) - BSA Free [NBP2-68006]

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) - BSA Free [NBP2-68006]

Flow Cytometry: HLA-ABCE Antibody (TP25.99SF) [NBP2-68006] - Staining of human peripheral blood cells using anti-HLA-ABCE (TP25.99SF) purified, GAM-APC.
HLA-ABCE Antibody (TP25.99SF) - BSA Free

Western Blot: HLA-ABCE Antibody (TP25.99SF) - BSA Free [NBP2-68006] -

Neonatal skin fibroblasts primed with inflammatory signals express less molecules that activate the immune system. (A) Schematic of the protocol used to stimulate neonatal (NSFs) and adult (ASFs) skin fibroblasts. (B) Western blots on ASF and NSF lysates for the detection of HLA-ABCE, HLA-DPDQDR, B7-H3 and alpha-tubulin (used as a loading control). (C) Densitometry quantifications normalized to alpha-tubulin. Statistics: Wald test with Tukey’s multiple-comparison tests; * p < 0.05, ** p < 0.01, *** p < 0.001, N = 6 different fibroblast populations (3 NSFs and 3 ASFs). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/13/6965), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Western Blot

1-2 µg/m
Application Notes
Western blotting: Recommended dilution: 1-2 ug/ml; non-reducing conditions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: HLA-ABCE

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.

Alternate Names

HLA-ABC, HLA-E, major histocompatibility complex, class I, A, major histocompatibility complex, class I, B, major histocompatibility complex, class I, C, major histocompatibility complex, class I, E, MHC class I

Additional HLA-ABCE Products

Product Documents for HLA-ABCE Antibody (TP25.99SF) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA-ABCE Antibody (TP25.99SF) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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