HLA A Antibody (8L2H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15377

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15377-100ul
NBP3-15377-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8L2H2 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HLA A Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA A (P04439). MAVMAPRTLLLLLSGALALTQTWAGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDQETRNVKAQSQTDRV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HLA A Antibody (8L2H2) (NBP3-15377) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HLA A Antibody (8L2H2)

Western Blot: HLA A Antibody (8L2H2) [NBP3-15377]

Western Blot: HLA A Antibody (8L2H2) [NBP3-15377]

Western Blot: HLA A Antibody (8L2H2) [NBP3-15377] - Western blot analysis of extracts of various cell lines, using HLA A antibody (NBP3-15377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: HLA A Antibody (8L2H2) [NBP3-15377]

Immunohistochemistry-Paraffin: HLA A Antibody (8L2H2) [NBP3-15377]

Immunohistochemistry-Paraffin: HLA A Antibody (8L2H2) [NBP3-15377] - Immunohistochemistry of paraffin-embedded human esophageal using HLA A Rabbit mAb (NBP3-15377) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation: HLA A Antibody (8L2H2) [NBP3-15377]

Immunoprecipitation: HLA A Antibody (8L2H2) [NBP3-15377]

Immunoprecipitation: HLA A Antibody (8L2H2) [NBP3-15377] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HLA A antibody (NBP3-15377). Western blot was performed from the immunoprecipitate using HLA A antibody (NBP3-15377) at a dilition of 1:2000.
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Applications for HLA A Antibody (8L2H2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA A

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Gene Symbol

HLA-A

Additional HLA A Products

Product Documents for HLA A Antibody (8L2H2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HLA A Antibody (8L2H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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