HIV-1 Gag p24 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB73601
Conjugate
Catalog #
Key Product Details
Species Reactivity
Viral
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 749138
Product Specifications
Immunogen
E. coli-derived recombinant viral HIV-1 Gag p24
Pro149-Gly354
Accession # Q77YG1_9HIV1
Pro149-Gly354
Accession # Q77YG1_9HIV1
Specificity
Detects viral HIV-1 Gag p24 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for HIV-1 Gag p24 Antibody
Detection of HIV‑1 Gag p24 by Western Blot.
Western blot shows lysates of recombinant HIV-1 Gag p24. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti- HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Viral HIV‑1 Gag p24 ELISA Standard Curve.
Recombinant Viral HIV-1 Gag p24 protein was serially diluted 2-fold and captured by Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for HIV-1 Gag p24 Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the HIV-1 Gag p24 DuoSet ELISA Kit (Catalog # DY7360-05) for convenient development of a sandwich ELISA or the HIV-1 Gag p24 Quantikine ELISA Kit (Catalog # DHP240) for a complete optimized ELISA.
Western Blot
0.1 µg/mL
Sample: Recombinant HIV-1 Gag p24
Sample: Recombinant HIV-1 Gag p24
Reviewed Applications
Read 1 review rated 5 using MAB73601 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIV-1 Gag p24
Long Name
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Alternate Names
CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)
Entrez Gene IDs
155030 (Viral)
Gene Symbol
GAG
UniProt
Additional HIV-1 Gag p24 Products
Product Documents for HIV-1 Gag p24 Antibody
Product Specific Notices for HIV-1 Gag p24 Antibody
For research use only
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