Histone H4 [ac Lys5] Antibody (23GB6055)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50453

Catalog #
Availability
Size / Price
Qty
NBP3-50453-100ul
NBP3-50453-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6055

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H4 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Histone H4 (acetyl K5) (Uniprot: P62805)

Modification

ac Lys5

Specificity

This antibody detects endogenous levels of H4C3 only when Acetylated at K5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [ac Lys5] Antibody (23GB6055)

Histone H4 [ac Lys5] Antibody (23GB6055)

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys5] Antibody (23GB6055)[NBP3-50453] -

Immunocytochemical staining of HepG2 cells with Histone H4 antibody (1:1,000). Nuclei were stained blue with DAPI; Histone H4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Histone H4 [ac Lys5] Antibody (23GB6055)

Flow Cytometry: Histone H4 [ac Lys5] Antibody (23GB6055)[NBP3-50453] -

Flow cytometric analysis of Histone H4expression in HepG2 cells using Histone H4 antibody (1:2,000). Green, isotype control; red, Histone H4 (acetyl K5).
Histone H4 [ac Lys5] Antibody (23GB6055)

Western Blot: Histone H4 [ac Lys5] Antibody (23GB6055)[NBP3-50453] -

Western blotting analysis using Histone H4 (acetyl K5) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Histone H4 [ac Lys5] Antibody (23GB6055)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys5] Antibody (23GB6055)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Histone H4 [ac Lys5] Antibody (23GB6055)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.