Histone H3.3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14981

Discontinued Product
NBP2-14981 has been discontinued. View all Histone H3.3 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Plant

Predicted:

Bovine (100%), Monkey (100%), Porcine (100%), Rabbit (100%), Xenopus (100%), Zebrafish (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Histone H3.3 Antibodies »

Product Specifications

Immunogen

Full length human Histone H3.3 Recombinant protein.

Reactivity Notes

Rice (100%), Xenopus laevis (100%).

Marker

Marker of Transcriptionally Active Chromatin

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3.3 Antibody

Western Blot: Histone H3.3 Antibody [NBP2-14981]

Western Blot: Histone H3.3 Antibody [NBP2-14981]

Western Blot: Histone H3.3 Antibody [NBP2-14981] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Immunocytochemistry/ Immunofluorescence: Histone H3.3 Antibody [NBP2-14981]

Immunocytochemistry/ Immunofluorescence: Histone H3.3 Antibody [NBP2-14981]

Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3.3 stained by Histone H3.3 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981]

Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981]

Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-14981] - Histone H3.3 antibody detects Histone H3.3 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H3.3 stained by Histone H3.3 antibody diluted at 1:500.
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Applications for Histone H3.3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Application Notes
ICC/IF-Assay dependent

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 20% Glycerol (pH7).

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Histone H3.3

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]

Alternate Names

H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783

Gene Symbol

H3-3A

Additional Histone H3.3 Products

Product Documents for Histone H3.3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Histone H3.3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.
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