Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57881
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Mouse (100%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL5245
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This antibody was developed using a synthetic peptide derived from O75367, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free
Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free
Analysis in human cell line HEL.
Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free
Analysis in human cell line HEL.
Histone H2AY/macroH2A.1 Antibody (CL5245) - BSA Free
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Applications for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2AY
Long Name
H2A Histone Family, Member Y
Alternate Names
H2AF12M, H2AFJ, H2AFY, Histone MacroH2A1, Medulloblastoma Antigen MU-MB-50.205
Gene Symbol
MACROH2A1
Additional Histone H2AY Products
Product Documents for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free
Product Specific Notices for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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