Histone H2AX Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21280
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (92%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PKKTSATVGPKAPSGGKKATQASQEY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AX Antibody - BSA Free
![Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21280] -](https://resources.bio-techne.com/images/products/nbp3-21280_rabbit-histone-h2ax-pab-45202315534326.jpg "Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21280] -")
Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-21280] -
Staining of human cell line A-431 shows localization to nucleoplasm & nuclear speckles.![Western Blot: Histone H2AX Antibody [NBP3-21280] -](https://resources.bio-techne.com/images/products/nbp3-21280_rabbit-histone-h2ax-pab-45202315493228.jpg "Western Blot: Histone H2AX Antibody [NBP3-21280] -")
Western Blot: Histone H2AX Antibody [NBP3-21280] -
Analysis in human cell line RT-4 and human cell line U-251 MG.Applications for Histone H2AX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody - BSA Free
Product Specific Notices for Histone H2AX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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