Histone H2AX Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04113
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-143 of human Histone H2AX (NP_002096.1). MSGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H2AX Antibody - BSA Free
![Western Blot: Histone H2AX AntibodyBSA Free [NBP3-04113]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-Western-Blot-NBP3-04113-img0003.jpg "Western Blot: Histone H2AX AntibodyBSA Free [NBP3-04113]")
Western Blot: Histone H2AX AntibodyBSA Free [NBP3-04113]
Western Blot: Histone H2AX Antibody [NBP3-04113] - Analysis of extracts of various cell lines, using Histone H2Ax antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody - BSA Free [Histone H2AX] -
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody - BSA Free [Histone H2AX] - Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunohistochemistry: Histone H2AX Antibody - BSA Free [Histone H2AX] -
Immunohistochemistry: Histone H2AX Antibody - BSA Free [Histone H2AX] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Histone H2AX Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Applications for Histone H2AX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50-1:100
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody - BSA Free
Product Specific Notices for Histone H2AX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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