Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-384MFV610
![Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE]](https://resources.bio-techne.com/images/products/nb100-384mfv610_rabbit-histone-h2ax-p-ser139-pab-mfluor-violet-610-se-672023994364.png "Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE] [NB100-384MFV610] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine
Applications
Immunohistochemistry, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)
Label
mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Histone H2AX [p Ser139] Antibody was developed against to a region surrounding phosphorylated serine 139 of human histone H2AX [Swiss-Prot entry P16104] (GeneID 3014).
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 27102221), Canine reactivity reported in scientific literature (PMID: 23365434).
Modification
p Ser139
Localization
Nucleous, chromosome.
Specificity
The epitope maps to a region surrounding phosphorylated serine 139 of human histone H2AX.
Marker
DNA Double-strand break marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE]
Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE] [NB100-384MFV610] -
Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE] [NB100-384MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.Applications for Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE]
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB100-384. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE]
Product Specific Notices for Histone H2AX [p Ser139] Antibody [mFluor Violet 610 SE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...