Histone H2AX Antibody (S02-3C8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20068
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S02-3C8 expressed in HEK293
Concentration
0.3 mg/ml
Product Specifications
Immunogen
A synthetic peptide of human Histone H2AX (Uniprot # P16104)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Histone H2AX Antibody (S02-3C8) (NBP3-20068) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Histone H2AX Antibody (S02-3C8)
![Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-S02-3C8-Western-Blot-NBP3-20068-img0002.jpg "Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068]")
Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068]
Western Blot: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Western blot detection of Histone H2AX in Hela, Ramos using NBP3-20068 (1:1000 diluted)![Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-S02-3C8-Immunohistochemistry-Paraffin-NBP3-20068-img0001.jpg "Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068]")
Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068]
Immunohistochemistry-Paraffin: Histone H2AX Antibody (S02-3C8) [NBP3-20068] - Immunohistochemistry of Histone H2AX in paraffin-embedded Human lung cancer tissue using NBP3-20068 at dilution 1/50Applications for Histone H2AX Antibody (S02-3C8)
Application
Recommended Usage
Immunohistochemistry
1/50
Western Blot
1/1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (S02-3C8)
Product Specific Notices for Histone H2AX Antibody (S02-3C8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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