Histone H2AX [p Ser139] Antibody (RM224)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18533
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Vertebrate
Applications
Multiplex Immunofluorescence, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM224 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
A phospho-peptide corresponding to Histone H2AX [Ser139]
Modification
p Ser139
Specificity
This antibody reacts to Histone H2AX only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AX [p Ser139] Antibody (RM224)
![Histone H2AX [p Ser139] Antibody (RM224)](https://resources.bio-techne.com/images/products/nbp3-18533_rabbit-histone-h2ax-p-ser-139-mab-rm224-immunocytochemistry-immunofluorescence-189202523444711.png "Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] -")
Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] -
Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-18533] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2AX [p Ser139] Antibody (RM224)
Application
Recommended Usage
ELISA
0.2 ug/mL - 1 ug/mL
Immunocytochemistry/ Immunofluorescence
0.5 ug/mL - 2 ug/mL
Western Blot
0.5 ug/mL - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX [p Ser139] Antibody (RM224)
Product Specific Notices for Histone H2AX [p Ser139] Antibody (RM224)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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