Histone H2AX Antibody (RM214) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26044
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Vertebrate
Applications
Multiplex Immunofluorescence, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM214 expressed in HEK293
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A peptide corresponding to the C-terminus of human Histone H2AX
Specificity
This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Histone H2AX Antibody (RM214) - Azide and BSA Free (NBP3-18534) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Histone H2AX Antibody (RM214) - Azide and BSA Free
Western Blot: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] -
Western Blot: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] - Western Blot of HeLa, HEK293, A375, and SK-MEL-2 whole cell lysates, using RM214 at 0.5 ug/mL, showed endogenous Histone H2AX in HeLa, HEK293, A375, and SK-MEL-2 cells.
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] -
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-26044] - Immunocytochemistry of HeLa cells, using Clone RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2AX Antibody (RM214) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/ml - 1 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/mL - 2 ug/ml
Western Blot
0.5 ug/mL - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (RM214) - Azide and BSA Free
Product Specific Notices for Histone H2AX Antibody (RM214) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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