Histone H2AX Antibody (CL5157) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44279

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL5157

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Histone H2AX Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P16104, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse Histone H2AX Antibody (CL5157) - Azide and BSA Free (NBP3-21157) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Histone H2AX Antibody (CL5157) - Azide and BSA Free

Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]

Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.
Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]

Staining of human kidney shows strong nuclear positivity in renal tubules cells.
Histone H2AX Antibody (CL5157) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]

Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-44279]

Staining of human endometrium shows strong nuclear immunoreactivity in glandular and stromal cells.
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Applications for Histone H2AX Antibody (CL5157) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20000 - 1:50000
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (CL5157) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2AX Antibody (CL5157) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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