Histone H2AX Antibody (BLR059F)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14680
Recombinant Monoclonal
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR059F
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
between 93 and 143 (C-term)
Clonality
Monoclonal
Host
Rabbit
Description
Novus Biologicals Rabbit Histone H2AX Antibody (BLR059F) (NBP3-14680) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Histone H2AX Antibody (BLR059F)
Western Blot;Histone H2AX Antibody (BLR059F)[NBP3-14680]
Western Blot;Histone H2AX Antibody (BLR059F) Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, RKO, LNCaP, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-H2AX recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin.Applications for Histone H2AX Antibody (BLR059F)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (BLR059F)
Product Specific Notices for Histone H2AX Antibody (BLR059F)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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