Histone H2a [ac Lys9] Antibody (23GB6080)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50455

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NBP3-50455-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6080

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2A Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Histone H2a (acetyl K9) (Uniprot: P04908)

Modification

ac Lys9

Specificity

This antibody detects endogenous levels of H2AC4 only when Acetylated at K9

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2a [ac Lys9] Antibody (23GB6080)

Histone H2a [ac Lys9] Antibody (23GB6080)

Immunocytochemistry/ Immunofluorescence: Histone H2a [ac Lys9] Antibody (23GB6080)[NBP3-50455] -

Immunocytochemical staining of Hela cells with Histone H2a antibody (1:1,000). Nuclei were stained blue with DAPI; Histone H2a was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Histone H2a [ac Lys9] Antibody (23GB6080)

Flow Cytometry: Histone H2a [ac Lys9] Antibody (23GB6080)[NBP3-50455] -

Flow cytometric analysis of Histone H2aexpression in C2C12 cells using Histone H2a antibody (1:2,000). Green, isotype control; red, Histone H2A (acetyl K9).
Histone H2a [ac Lys9] Antibody (23GB6080)

Western Blot: Histone H2a [ac Lys9] Antibody (23GB6080)[NBP3-50455] -

Western blotting analysis using Histone H2a (acetyl K9) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Histone H2a [ac Lys9] Antibody (23GB6080)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2A

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Long Name

H2A Histone Family

Alternate Names

H2A1B, HIST1H2AB

Gene Symbol

H2AC20

Additional Histone H2A Products

Product Documents for Histone H2a [ac Lys9] Antibody (23GB6080)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H2a [ac Lys9] Antibody (23GB6080)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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