HIGD1A Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03930
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2). MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit HIGD1A Antibody - Azide and BSA Free (NBP3-03930) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HIGD1A Antibody - Azide and BSA Free
![Western Blot: HIGD1A AntibodyAzide and BSA Free [NBP3-03930]](https://resources.bio-techne.com/images/products/HIGD1A-Antibody-Western-Blot-NBP3-03930-img0003.jpg "Western Blot: HIGD1A AntibodyAzide and BSA Free [NBP3-03930]")
Western Blot: HIGD1A AntibodyAzide and BSA Free [NBP3-03930]
Western Blot: HIGD1A Antibody [NBP3-03930] - Analysis of extracts of various cell lines, using HIGD1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.![Immunocytochemistry/ Immunofluorescence: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]](https://resources.bio-techne.com/images/products/HIGD1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03930-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]")
Immunocytochemistry/ Immunofluorescence: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]
Immunocytochemistry/Immunofluorescence: HIGD1A Antibody [NBP3-03930] - Analysis of U-2 OS cells using HIGD1A antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]](https://resources.bio-techne.com/images/products/HIGD1A-Antibody-Immunohistochemistry-Paraffin-NBP3-03930-img0002.jpg "Immunohistochemistry-Paraffin: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]")
Immunohistochemistry-Paraffin: HIGD1A Antibody - Azide and BSA Free [NBP3-03930]
Immunohistochemistry-Paraffin: HIGD1A Antibody [NBP3-03930] - Human colon carcinoma using HIGD1A antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for HIGD1A Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50-1:100
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HIGD1A
Alternate Names
7420700H20Rik, DKFZP564K247, HIG1 domain family member 1A, HIG1 domain family, member 1A, HIG1 hypoxia inducible domain family, member 1A, HIG1DKFZp564K247, hypoxia inducible gene 1,2210020B17Rik, Hypoxia-inducible gene 1 protein
Gene Symbol
HIGD1A
Additional HIGD1A Products
Product Documents for HIGD1A Antibody - Azide and BSA Free
Product Specific Notices for HIGD1A Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov