Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43581
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing BALB/c mice with synthetic N-terminal glycopeptide (VHLTPEC) and KLH conjugate. (Uniprot# P68871)
Specificity
This antibody binds human hemoglobin glycated at the N-terminal valine of the hemoglobin beta chain and does not cross react with non-glycated hemoglobin.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This is a chimeric antibody made using the variable domain sequences of the original Mouse IgG2a to improve compatibility with existing reagents, assays, and techniques.
Scientific Data Images for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free
Western Blot: Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free [NBP3-43581] -
Western Blot: Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free [NBP3-43581] - Human liver (A) (0.01ug/ml), heart (B) (0.01ug/ml), placenta (C) (0.01ug/ml), and lungs (D) (0.003ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43581 at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.Applications for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Hemoglobin beta
Alternate Names
beta globin chain, beta-globin, CD113t-C, HBBB, HBD, Hemoglobin beta chain, hemoglobin subunit beta, hemoglobin, beta
Gene Symbol
HBB
Additional Hemoglobin beta Products
Product Documents for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free
Product Specific Notices for Hemoglobin beta Antibody (1G8) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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