HE4/WFDC2 Antibody (JF62-09)

Immunocytochemistry/Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] -

Immunocytochemistry/Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HepG2 cells labeling HE4 with Rabbit anti-HE4 antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HE4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4c. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.