HA Tag Antibody (RMH02) - Chimeric
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61476
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
Western Blot, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # RMH02 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
This HA Tag Antibody (RMH02) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106). This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of NBP2-61477 with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.
Specificity
This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Description
Novus Biologicals Human HA Tag Antibody (RMH02) - Chimeric (NBP2-61476) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Applications for HA Tag Antibody (RMH02) - Chimeric
Application
Recommended Usage
Sandwich ELISA Capture
0.5 - 2 ug/ml
Sandwich ELISA Detection
0.01 - 0.5 ug/ml
Western Blot
0.1 ug/ml - 1 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Documents for HA Tag Antibody (RMH02) - Chimeric
Product Specific Notices for HA Tag Antibody (RMH02) - Chimeric
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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