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GRPEL1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04531

Novus Biologicals, part of Bio-Techne
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NBP3-04531-100ul
NBP3-04531-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRPEL1 (NP_079472.1). MAAQCVRLARRSLPALALSLRPSPRLLCTATKQKNSGQNLEEDMGQSEQKADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAKLYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GRPEL1 Antibody - BSA Free (NBP3-04531) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GRPEL1 Antibody - BSA Free

GRPEL1 Antibody - BSA Free

Western Blot: GRPEL1 Antibody - BSA Free [NBP3-04531] -

Western Blot: GRPEL1 Antibody - BSA Free [NBP3-04531] - Western blot analysis of lysates from Mouse liver, using GRPEL1 Rabbit pAb (A4999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
GRPEL1 Antibody - BSA Free

Immunohistochemistry: GRPEL1 Antibody - BSA Free [NBP3-04531] -

Immunohistochemistry: GRPEL1 Antibody - BSA Free [NBP3-04531] - Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human tonsil tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
GRPEL1 Antibody - BSA Free

Immunohistochemistry: GRPEL1 Antibody - BSA Free [NBP3-04531] -

Immunohistochemistry: GRPEL1 Antibody - BSA Free [NBP3-04531] - Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human liver tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for GRPEL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRPEL1

Essential component of the PAM complex, a complex required for the translocation of transitpeptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems tocontrol the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins

Alternate Names

FLJ25609, GREPEL1, grpE protein homolog 1, mitochondrial, GrpE-like 1, mitochondrial (E. coli), GrpE-like protein cochaperone, HMGEmt-GrpE#1, Mt-GrpE#1

Gene Symbol

GRPEL1

Additional GRPEL1 Products

Product Documents for GRPEL1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRPEL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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