GRPEL1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83557

Novus Biologicals, part of Bio-Techne
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NBP1-83557-25ul
NBP1-83557
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRPEL1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRPEL1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GRPEL1 Antibody [NBP1-83557]

Immunocytochemistry/ Immunofluorescence: GRPEL1 Antibody [NBP1-83557]

Immunocytochemistry/Immunofluorescence: GRPEL1 Antibody [NBP1-83557] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.
GRPEL1 Antibody - BSA Free Immunohistochemistry-Paraffin: GRPEL1 Antibody - BSA Free [NBP1-83557]

Immunohistochemistry-Paraffin: GRPEL1 Antibody - BSA Free [NBP1-83557]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
GRPEL1 Antibody - BSA Free Immunohistochemistry-Paraffin: GRPEL1 Antibody - BSA Free [NBP1-83557]

Immunohistochemistry-Paraffin: GRPEL1 Antibody - BSA Free [NBP1-83557]

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
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Applications for GRPEL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRPEL1

Essential component of the PAM complex, a complex required for the translocation of transitpeptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems tocontrol the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins

Alternate Names

FLJ25609, GREPEL1, grpE protein homolog 1, mitochondrial, GrpE-like 1, mitochondrial (E. coli), GrpE-like protein cochaperone, HMGEmt-GrpE#1, Mt-GrpE#1

Gene Symbol

GRPEL1

Additional GRPEL1 Products

Product Documents for GRPEL1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRPEL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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